Original Research
Saturday, November 11, 2023
11:30 AM–01:00 PM
Abstract
Introduction:
Whether microRNAs (miRNAs) from plasma exosomes might be dysregulated in patients with depression, especially treatment-resistant depression (TRD), remains unclear, based on the study of which novel biomarkers and therapeutic targets could be discovered.
Research Question or Hypothesis: To this end, a small sample study was performed by isolation of plasma exosomes from patients with TRD diagnosed by the Hamilton scale.
Study Design: In this study, 4 peripheral plasma samples from patients with TRD and 4 healthy controls were collected for extraction of plasma exosomes.
Methods: Exosomal miRNAs were analyzed by miRNA sequencing, followed by image collection, expression difference analysis, target gene GO enrichment analysis, and KEGG pathway enrichment analysis.
Results: Compared with the healthy controls, 2 miRNAs in the plasma exosomes of patients with TRD showed significant differences in expression, among which has-miR-335-5p were significantly upregulated and has-miR-1292-3p were significantly downregulated. Go and KEGG analysis showed that dysregulated miRNAs affect postsynaptic density and axonogenesis as well as the signaling pathway of axon formation and cell growths.
Conclusion: The identification of these miRNAs and their target genes may provide novel biomarkers for improving diagnostic accuracy and treatment effectiveness of TRD.
Presenting Author
Muhammad Naveed PharmD, PhD.Nanjing Medical University
Authors