Original Research
Sunday, November 12, 2023
12:45 PM–02:15 PM
Abstract
Introduction: Air pollution continues to be a major public health concern affecting nine out of ten individuals living in urban areas worldwide. Ultrafine particles (UFP) which is known to be the smallest airborne particles with a diameter of <100 nanometers are emitted from natural sources such as wood burning and cigarette smoking. Chronic obstructive pulmonary disease COPD was found to be aggravated by exposure to a variety of air pollutants, resulting in senescence. Studies have connected exposure of UFP to accelerated lung ageing owing to an increase in the number of senescent cells. Research Question or Hypothesis:The purpose of the study was to examine the impact of UFP (Fe(OH)3, AgCl and Polystyrene) exposure on the progression of senescence in airway bronchial epithelial cells.
Study Design: Two different assays were employed for this study, MTT and senescence beta-galactosidase staining assay.
Methods: The in vitro exposure of 16HBE14o- cell process included three different concentrations for Fe(OH)3 and polystyrene: 7.81, 15.62 and 125µg/mL; two concentrations were examine for AgCl: 7.81 and 15.62µg/mL for 24 hours.
Results: Our results indicated that the impact on cellular viability revealed that UFP was cytotoxic in a dose-dependent manner. Additionally, beta-galactosidase proportionally increased with UFP concentration compared to untreated cells, indicating that cellular senescence was promoted in all UFP concentration.
Conclusion: Low-dose ultrafine particle (Fe(OH)3, AgCl and polystyrene) exposure for 24 hours and seven days induced senescence in airway epithelial cells in vitro. Therefore, daily intake of ultrafine particles released into the air is a risk factor and potential mediator for promoting senescence and COPD exacerbation. Absence of zeta-potential measurement was a limitation to this study as aggregations and surface charges of the UFP were not measured. Future research is required to identify the mechanism and type of stress induced by these particles should be explored and identified in greater detail.
Presenting Author
Ban Alwali MSc Clinical PharmacyUniversity of Brighton
Authors